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ralav23  (Addgene inc)


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    Addgene inc ralav23
    Figure4. RAS/ERKsignalinginhibitscorticalneurogenesis.A–L,E12.53E15.5electroporationsofapCIG2controlvector(A,A,E,E,I,I),RasV12(B,B,F,F,J,J),bRafV600E(C,C,G,G, K,K),andMekCA(D,D,H,H,L,L)vectors(expressingGFP).TransfectedbrainswereanalyzedforcoexpressionofGFP(green)withpan-neuronalmarkerNeuN(red;A–D,A–D)andthecortical neuronal marker Tbr1 (red; E–H, E–H), or for transcripts for GFP (I–L) or the subcortical neuronal marker Dlx1 (I–L). Dashed lines outline the transfected region in the neocortex. Yellow arrowheads mark transfected areas with reduced expression of NeuN (B, C, D), and Tbr1 (F, G, H), and red arrowheads mark ectopic expression of Dlx1 (L). Scale bars: 250 m. M, N, QuantificationofGFP <t>cellscoexpressingNeuN(M)orTbr1(N).pvaluesofpCIG2-RasV12,pEF-RasV12,bRafV600E,MekCA,AktCA,RalAV23,andRalBQ72EarerelativetopCIG2control,whereasp</t> values of pCIG2RasV12pCIG2MekDN, pCIG2RasV12pCIG2AktDN, pCIG2Rasv12pCIG2RalADN, and pCIG2RasV12pCIG2RalBDN are relative to pCIG2RasV12. *p 0.05, **p 0.01, ***p 0.005. ctx, neocortex; str, striatum.
    Ralav23, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ralav23/product/Addgene inc
    Average 91 stars, based on 5 article reviews
    ralav23 - by Bioz Stars, 2026-06
    91/100 stars

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    1) Product Images from "RAS/ERK Signaling Controls Proneural Genetic Programs in Cortical Development and Gliomagenesis"

    Article Title: RAS/ERK Signaling Controls Proneural Genetic Programs in Cortical Development and Gliomagenesis

    Journal: Journal of Neuroscience

    doi: 10.1523/jneurosci.4077-13.2014

    Figure4. RAS/ERKsignalinginhibitscorticalneurogenesis.A–L,E12.53E15.5electroporationsofapCIG2controlvector(A,A,E,E,I,I),RasV12(B,B,F,F,J,J),bRafV600E(C,C,G,G, K,K),andMekCA(D,D,H,H,L,L)vectors(expressingGFP).TransfectedbrainswereanalyzedforcoexpressionofGFP(green)withpan-neuronalmarkerNeuN(red;A–D,A–D)andthecortical neuronal marker Tbr1 (red; E–H, E–H), or for transcripts for GFP (I–L) or the subcortical neuronal marker Dlx1 (I–L). Dashed lines outline the transfected region in the neocortex. Yellow arrowheads mark transfected areas with reduced expression of NeuN (B, C, D), and Tbr1 (F, G, H), and red arrowheads mark ectopic expression of Dlx1 (L). Scale bars: 250 m. M, N, QuantificationofGFP cellscoexpressingNeuN(M)orTbr1(N).pvaluesofpCIG2-RasV12,pEF-RasV12,bRafV600E,MekCA,AktCA,RalAV23,andRalBQ72EarerelativetopCIG2control,whereasp values of pCIG2RasV12pCIG2MekDN, pCIG2RasV12pCIG2AktDN, pCIG2Rasv12pCIG2RalADN, and pCIG2RasV12pCIG2RalBDN are relative to pCIG2RasV12. *p 0.05, **p 0.01, ***p 0.005. ctx, neocortex; str, striatum.
    Figure Legend Snippet: Figure4. RAS/ERKsignalinginhibitscorticalneurogenesis.A–L,E12.53E15.5electroporationsofapCIG2controlvector(A,A,E,E,I,I),RasV12(B,B,F,F,J,J),bRafV600E(C,C,G,G, K,K),andMekCA(D,D,H,H,L,L)vectors(expressingGFP).TransfectedbrainswereanalyzedforcoexpressionofGFP(green)withpan-neuronalmarkerNeuN(red;A–D,A–D)andthecortical neuronal marker Tbr1 (red; E–H, E–H), or for transcripts for GFP (I–L) or the subcortical neuronal marker Dlx1 (I–L). Dashed lines outline the transfected region in the neocortex. Yellow arrowheads mark transfected areas with reduced expression of NeuN (B, C, D), and Tbr1 (F, G, H), and red arrowheads mark ectopic expression of Dlx1 (L). Scale bars: 250 m. M, N, QuantificationofGFP cellscoexpressingNeuN(M)orTbr1(N).pvaluesofpCIG2-RasV12,pEF-RasV12,bRafV600E,MekCA,AktCA,RalAV23,andRalBQ72EarerelativetopCIG2control,whereasp values of pCIG2RasV12pCIG2MekDN, pCIG2RasV12pCIG2AktDN, pCIG2Rasv12pCIG2RalADN, and pCIG2RasV12pCIG2RalBDN are relative to pCIG2RasV12. *p 0.05, **p 0.01, ***p 0.005. ctx, neocortex; str, striatum.

    Techniques Used: Marker, Transfection, Expressing

    Figure 6. Activation of RAS signaling in cortical progenitors results in tumorigenesis. A–E, E18.5 control (C) and E12.53E18.5 brains electroporated with pCIG2RasV12 (A, B, D, E, E) were processed for H&E staining (B–E, E), or GFP transcripts (A). F–H, E12.53E16.5 electroporations of a pCIG2 control vector (F–F) and pCIG2RasV12 (G–G, H–H). Transfected brains were analyzed for coexpression of GFP (green), proliferation marker Ki67 (red), and pan-neuronal marker Tuj1 (blue). White arrowheads mark RasV12 transfected cells expressing Ki67 instead of Tuj1 (H–H).I–P,E12.53E15.5brainselectroporatedwithapCIG2controlvector(I,I,K,K,M,M,O,O)orpCIG2RasV12(J,J,L,L,N,N,P,P)wereprocessedforimmunostainingofpan-neural progenitormarkersSox2(I,I,J,J)andNestin(K,K,L,L),cortical-specificprogenitormarkersPax6(M,M,N,N),andTbr2(O,O,P,P).RedarrowheadsmarkectopicexpressionofSox2(J) and Nestin (L), while yellow arrowheads mark transfected areas with reduced expression of Pax6 (N) and Tbr2 (P). Q–V, E12.53E15.5 electroporations of pCIG2RasV12 with (T–V, V) or withoutNeurog2(Q–S,S).TransfectedbrainswereanalyzedforthetranscriptsofSpry2(Q,T),Pdgfa(R,U),orcoexpressionofGFPandTuj1(S,S,V,V).Redarrowheadsmarkectopicexpression ofSpry2(Q,T)orPdgfa(R).W–Z,E12.53E15.5electroporationsofpCIG2(W,W),AktA(X,X),RalAV23(Y,Y),andRalBQ72L(Z,Z)followedbyanalysisofBrdUincorporation.Redarrowheads markectopicBrdUincorporation.Dashedlinesoutlinethetransfectedregionintheneocortex.Scalebars:I–V,I–V,F–F,G–G250m;H–H,67.5m.CP,corticalplate;ctx,neocortex;str,striatum.
    Figure Legend Snippet: Figure 6. Activation of RAS signaling in cortical progenitors results in tumorigenesis. A–E, E18.5 control (C) and E12.53E18.5 brains electroporated with pCIG2RasV12 (A, B, D, E, E) were processed for H&E staining (B–E, E), or GFP transcripts (A). F–H, E12.53E16.5 electroporations of a pCIG2 control vector (F–F) and pCIG2RasV12 (G–G, H–H). Transfected brains were analyzed for coexpression of GFP (green), proliferation marker Ki67 (red), and pan-neuronal marker Tuj1 (blue). White arrowheads mark RasV12 transfected cells expressing Ki67 instead of Tuj1 (H–H).I–P,E12.53E15.5brainselectroporatedwithapCIG2controlvector(I,I,K,K,M,M,O,O)orpCIG2RasV12(J,J,L,L,N,N,P,P)wereprocessedforimmunostainingofpan-neural progenitormarkersSox2(I,I,J,J)andNestin(K,K,L,L),cortical-specificprogenitormarkersPax6(M,M,N,N),andTbr2(O,O,P,P).RedarrowheadsmarkectopicexpressionofSox2(J) and Nestin (L), while yellow arrowheads mark transfected areas with reduced expression of Pax6 (N) and Tbr2 (P). Q–V, E12.53E15.5 electroporations of pCIG2RasV12 with (T–V, V) or withoutNeurog2(Q–S,S).TransfectedbrainswereanalyzedforthetranscriptsofSpry2(Q,T),Pdgfa(R,U),orcoexpressionofGFPandTuj1(S,S,V,V).Redarrowheadsmarkectopicexpression ofSpry2(Q,T)orPdgfa(R).W–Z,E12.53E15.5electroporationsofpCIG2(W,W),AktA(X,X),RalAV23(Y,Y),andRalBQ72L(Z,Z)followedbyanalysisofBrdUincorporation.Redarrowheads markectopicBrdUincorporation.Dashedlinesoutlinethetransfectedregionintheneocortex.Scalebars:I–V,I–V,F–F,G–G250m;H–H,67.5m.CP,corticalplate;ctx,neocortex;str,striatum.

    Techniques Used: Activation Assay, Control, Staining, Plasmid Preparation, Transfection, Marker, Expressing



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    Figure4. RAS/ERKsignalinginhibitscorticalneurogenesis.A–L,E12.53E15.5electroporationsofapCIG2controlvector(A,A,E,E,I,I),RasV12(B,B,F,F,J,J),bRafV600E(C,C,G,G, K,K),andMekCA(D,D,H,H,L,L)vectors(expressingGFP).TransfectedbrainswereanalyzedforcoexpressionofGFP(green)withpan-neuronalmarkerNeuN(red;A–D,A–D)andthecortical neuronal marker Tbr1 (red; E–H, E–H), or for transcripts for GFP (I–L) or the subcortical neuronal marker Dlx1 (I–L). Dashed lines outline the transfected region in the neocortex. Yellow arrowheads mark transfected areas with reduced expression of NeuN (B, C, D), and Tbr1 (F, G, H), and red arrowheads mark ectopic expression of Dlx1 (L). Scale bars: 250 m. M, N, QuantificationofGFP <t>cellscoexpressingNeuN(M)orTbr1(N).pvaluesofpCIG2-RasV12,pEF-RasV12,bRafV600E,MekCA,AktCA,RalAV23,andRalBQ72EarerelativetopCIG2control,whereasp</t> values of pCIG2RasV12pCIG2MekDN, pCIG2RasV12pCIG2AktDN, pCIG2Rasv12pCIG2RalADN, and pCIG2RasV12pCIG2RalBDN are relative to pCIG2RasV12. *p 0.05, **p 0.01, ***p 0.005. ctx, neocortex; str, striatum.
    Ralav23, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ralav23/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    ralav23 - by Bioz Stars, 2026-06
    91/100 stars
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    Figure4. RAS/ERKsignalinginhibitscorticalneurogenesis.A–L,E12.53E15.5electroporationsofapCIG2controlvector(A,A,E,E,I,I),RasV12(B,B,F,F,J,J),bRafV600E(C,C,G,G, K,K),andMekCA(D,D,H,H,L,L)vectors(expressingGFP).TransfectedbrainswereanalyzedforcoexpressionofGFP(green)withpan-neuronalmarkerNeuN(red;A–D,A–D)andthecortical neuronal marker Tbr1 (red; E–H, E–H), or for transcripts for GFP (I–L) or the subcortical neuronal marker Dlx1 (I–L). Dashed lines outline the transfected region in the neocortex. Yellow arrowheads mark transfected areas with reduced expression of NeuN (B, C, D), and Tbr1 (F, G, H), and red arrowheads mark ectopic expression of Dlx1 (L). Scale bars: 250 m. M, N, QuantificationofGFP cellscoexpressingNeuN(M)orTbr1(N).pvaluesofpCIG2-RasV12,pEF-RasV12,bRafV600E,MekCA,AktCA,RalAV23,andRalBQ72EarerelativetopCIG2control,whereasp values of pCIG2RasV12pCIG2MekDN, pCIG2RasV12pCIG2AktDN, pCIG2Rasv12pCIG2RalADN, and pCIG2RasV12pCIG2RalBDN are relative to pCIG2RasV12. *p 0.05, **p 0.01, ***p 0.005. ctx, neocortex; str, striatum.

    Journal: Journal of Neuroscience

    Article Title: RAS/ERK Signaling Controls Proneural Genetic Programs in Cortical Development and Gliomagenesis

    doi: 10.1523/jneurosci.4077-13.2014

    Figure Lengend Snippet: Figure4. RAS/ERKsignalinginhibitscorticalneurogenesis.A–L,E12.53E15.5electroporationsofapCIG2controlvector(A,A,E,E,I,I),RasV12(B,B,F,F,J,J),bRafV600E(C,C,G,G, K,K),andMekCA(D,D,H,H,L,L)vectors(expressingGFP).TransfectedbrainswereanalyzedforcoexpressionofGFP(green)withpan-neuronalmarkerNeuN(red;A–D,A–D)andthecortical neuronal marker Tbr1 (red; E–H, E–H), or for transcripts for GFP (I–L) or the subcortical neuronal marker Dlx1 (I–L). Dashed lines outline the transfected region in the neocortex. Yellow arrowheads mark transfected areas with reduced expression of NeuN (B, C, D), and Tbr1 (F, G, H), and red arrowheads mark ectopic expression of Dlx1 (L). Scale bars: 250 m. M, N, QuantificationofGFP cellscoexpressingNeuN(M)orTbr1(N).pvaluesofpCIG2-RasV12,pEF-RasV12,bRafV600E,MekCA,AktCA,RalAV23,andRalBQ72EarerelativetopCIG2control,whereasp values of pCIG2RasV12pCIG2MekDN, pCIG2RasV12pCIG2AktDN, pCIG2Rasv12pCIG2RalADN, and pCIG2RasV12pCIG2RalBDN are relative to pCIG2RasV12. *p 0.05, **p 0.01, ***p 0.005. ctx, neocortex; str, striatum.

    Article Snippet: Huang, Addgene plasmid 15269; B. P. Zhou et al., 2000), RalAV23 (from W. Hahn, Addgene plasmid 15252; Sablina et al., 2007), RalADN (RalAS31N, from C. Counter, Addgene plasmids 19718; Lim et al., 2005), RalBQ72L (from C. Counter, Addgene plasmid 19721; Lim et al., 2005), and RalBDN (RalBS28N, from C. Counter, Addgene plasmid 19722; Lim et al., 2005) were obtained from Addgene.

    Techniques: Marker, Transfection, Expressing

    Figure 6. Activation of RAS signaling in cortical progenitors results in tumorigenesis. A–E, E18.5 control (C) and E12.53E18.5 brains electroporated with pCIG2RasV12 (A, B, D, E, E) were processed for H&E staining (B–E, E), or GFP transcripts (A). F–H, E12.53E16.5 electroporations of a pCIG2 control vector (F–F) and pCIG2RasV12 (G–G, H–H). Transfected brains were analyzed for coexpression of GFP (green), proliferation marker Ki67 (red), and pan-neuronal marker Tuj1 (blue). White arrowheads mark RasV12 transfected cells expressing Ki67 instead of Tuj1 (H–H).I–P,E12.53E15.5brainselectroporatedwithapCIG2controlvector(I,I,K,K,M,M,O,O)orpCIG2RasV12(J,J,L,L,N,N,P,P)wereprocessedforimmunostainingofpan-neural progenitormarkersSox2(I,I,J,J)andNestin(K,K,L,L),cortical-specificprogenitormarkersPax6(M,M,N,N),andTbr2(O,O,P,P).RedarrowheadsmarkectopicexpressionofSox2(J) and Nestin (L), while yellow arrowheads mark transfected areas with reduced expression of Pax6 (N) and Tbr2 (P). Q–V, E12.53E15.5 electroporations of pCIG2RasV12 with (T–V, V) or withoutNeurog2(Q–S,S).TransfectedbrainswereanalyzedforthetranscriptsofSpry2(Q,T),Pdgfa(R,U),orcoexpressionofGFPandTuj1(S,S,V,V).Redarrowheadsmarkectopicexpression ofSpry2(Q,T)orPdgfa(R).W–Z,E12.53E15.5electroporationsofpCIG2(W,W),AktA(X,X),RalAV23(Y,Y),andRalBQ72L(Z,Z)followedbyanalysisofBrdUincorporation.Redarrowheads markectopicBrdUincorporation.Dashedlinesoutlinethetransfectedregionintheneocortex.Scalebars:I–V,I–V,F–F,G–G250m;H–H,67.5m.CP,corticalplate;ctx,neocortex;str,striatum.

    Journal: Journal of Neuroscience

    Article Title: RAS/ERK Signaling Controls Proneural Genetic Programs in Cortical Development and Gliomagenesis

    doi: 10.1523/jneurosci.4077-13.2014

    Figure Lengend Snippet: Figure 6. Activation of RAS signaling in cortical progenitors results in tumorigenesis. A–E, E18.5 control (C) and E12.53E18.5 brains electroporated with pCIG2RasV12 (A, B, D, E, E) were processed for H&E staining (B–E, E), or GFP transcripts (A). F–H, E12.53E16.5 electroporations of a pCIG2 control vector (F–F) and pCIG2RasV12 (G–G, H–H). Transfected brains were analyzed for coexpression of GFP (green), proliferation marker Ki67 (red), and pan-neuronal marker Tuj1 (blue). White arrowheads mark RasV12 transfected cells expressing Ki67 instead of Tuj1 (H–H).I–P,E12.53E15.5brainselectroporatedwithapCIG2controlvector(I,I,K,K,M,M,O,O)orpCIG2RasV12(J,J,L,L,N,N,P,P)wereprocessedforimmunostainingofpan-neural progenitormarkersSox2(I,I,J,J)andNestin(K,K,L,L),cortical-specificprogenitormarkersPax6(M,M,N,N),andTbr2(O,O,P,P).RedarrowheadsmarkectopicexpressionofSox2(J) and Nestin (L), while yellow arrowheads mark transfected areas with reduced expression of Pax6 (N) and Tbr2 (P). Q–V, E12.53E15.5 electroporations of pCIG2RasV12 with (T–V, V) or withoutNeurog2(Q–S,S).TransfectedbrainswereanalyzedforthetranscriptsofSpry2(Q,T),Pdgfa(R,U),orcoexpressionofGFPandTuj1(S,S,V,V).Redarrowheadsmarkectopicexpression ofSpry2(Q,T)orPdgfa(R).W–Z,E12.53E15.5electroporationsofpCIG2(W,W),AktA(X,X),RalAV23(Y,Y),andRalBQ72L(Z,Z)followedbyanalysisofBrdUincorporation.Redarrowheads markectopicBrdUincorporation.Dashedlinesoutlinethetransfectedregionintheneocortex.Scalebars:I–V,I–V,F–F,G–G250m;H–H,67.5m.CP,corticalplate;ctx,neocortex;str,striatum.

    Article Snippet: Huang, Addgene plasmid 15269; B. P. Zhou et al., 2000), RalAV23 (from W. Hahn, Addgene plasmid 15252; Sablina et al., 2007), RalADN (RalAS31N, from C. Counter, Addgene plasmids 19718; Lim et al., 2005), RalBQ72L (from C. Counter, Addgene plasmid 19721; Lim et al., 2005), and RalBDN (RalBS28N, from C. Counter, Addgene plasmid 19722; Lim et al., 2005) were obtained from Addgene.

    Techniques: Activation Assay, Control, Staining, Plasmid Preparation, Transfection, Marker, Expressing